Genetic Tests

Lab	Molecular Haematology PaLMS-RNSH NSW
Test Name	Chimerism study for bone marrow transplant monitoring
Genes tested	FGA Chromosome_6 Chromosome_3 Chromosome_21 Chromosome_18 Chromosome_17 APOC2 AMELY
Test Description	Monitoring of bone marrow engraftment using apolipoprotein C-II, D3S1282, D3S1300, D6S264, D18S62, fibrinogen alpha chain, Penta D, Penta E, D18S51, D21S11, D17S5, amelogenin, Y-linked. Chimerism analysis cannot be performed unless a donor specimen and a pre-transplant recipient specimen have been received by the laboratory.
Method Type	specific assay/s
Method Comment	A pre-determined informative polymorphic genetic marker is used to determine the percentage of donor and recipient leucoytes in a post-transplant specimen using end-point PCR and denaturing polyacrylamide gel electrophoresis.
Sample Required	Must make booking with Flow Cytometry lab before collecting post-transplant samples. Donor: 2x10ml EDTA blood; Pre-transplant recipient: 2x10ml EDTA blood; Post-transplant recipient 1ml EDTA bone marrow RCPA guideline re sample requirements
Turn around	3 weeks

The following information is provided and updated from time to time by laboratories providing genetic testing. The list of laboratories is not an exhaustive list of the laboratories that provide such testing. Links to a third party website are provided for convenience only. The Royal College of Pathologists of Australasia (RCPA) accepts no responsibility for, and makes no representation concerning, the accuracy, appropriateness, and completeness of the information contained on the following pages or on any linked websites. The RCPA does not provide any direct or implied endorsement of the tests or laboratories listed, and accepts no responsibility for the information provided. The RCPA recommends that all medical testing be performed in an accredited laboratory, and emphasises that the listing of a test or laboratory on this page does not imply that it has been accredited by NATA/RCPA or any other organisation. Users of this information should seek expert professional advice before making any decisions based on this information. Please note that charges may apply for gene tests; enquiries about charges should be made to the laboratory to which a sample is to be referred prior to referral. Users access the linked websites at their own risk.

The Rotal College of Pathologists of Australia